The general problem on natural energy source in the world have been thought by many scientist and technologist to create new energy synthetic to modified other energy that come from plant can be used also as energy in industry and in our home, because the plant energy source can be renewed, while energy from mineral can’t be renewed. Many scientist have try to produce biodiesel from many source but just few of their experiment can be applied into the real production. Biodiesel Separation Several oil plant can be proceed into fuel oil through several process stage. Most of the animal fats, vegetables and oils contain glycerin and are thus called triglycerides can be proceed to make biofuels, all the fats and oils are turned into esters, then separating the glycerin. At the end of the process, all the glycerin sinks down at the bottom and all the biodiesel rests at the top. The process through which the glycerin is separated from the biodiesel is known as transesterification. Th